A Critical Appraisal of the Separation Protocols Proposed for the Implementation of the Health Claim on “Olive Oil Polyphenols” (EC Regulation 432/2012)

The analysis of the secoiridoid type phenolic compounds present in virgin olive oil has become a challenging area research since first evidence their presence polar fraction oil. Separation techniques, mainly liquid chromatographic ones, prevailed over years application toward elucidation structure, content determination and collection on cultivar, origin, processing storage conditions dependence. One latest challenges was related to need address requirement set by EC Regulation 432/2012 for implementation health claim ‘olive polyphenols’. work considers chronological order original articles, viewpoints, review articles other published efforts that appeared literature after issuing relevant EFSA scientific opinion 2011. created lot expectations among producers boosted development ‘fit purpose’ analytical protocol. Emphasis is given dedicated separation protocols have been developed last 10 progress validation comparison features method were recently adopted International Olive Council.